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K. Ravishankar ¹, Ch. Sandhya ²

Affiliations
1 Department of Pharmacology, VJ’s college of Pharmacy, Diwanchervu, Rajahmundry, East Godavari District, A.P, Pin code-533 103, IN
2 Department of Pharmacology, Sri Sai Aditya Institute of Pharmaceutical Sciences and Research, ADB Road, Surampalem, East Godavari District, A.P, Pin code-533 437, IN

Abstract

Herbal medicine has been used for many years by different cultures around the world for the treatment of diabetes and hence the alcoholic bark extract of Mimosa catechu (AEMC) Wild plant was investigated for its possible antihyperglycemic effect in alloxan induced diabetic rats. The animals were made diabetic by intraperitoneal injection of alloxan monohydrate at a dose of 150mg/kg body weight. The alcoholic extract of Mimosa catechu, AEMC (250 and 500mg kg^-1) and the standard drug (Glibenclamide 0.5mg/kg) were administered orally. Control group of rats were administered 0.2ml of 10% acacia mucilage (vehicle). The effect of oral administration of AEMC for 7days on the levels of Serum glucose, total cholesterol, and triglycerides, HDL-cholesterol, LDL-cholesterol in normal and diabetic rats were evaluated and compared with that of standard antidiabetic drug, Glibenclamide. Oral administration of 250 and 500mg kg^-1 body wt of AEMC for 7 days exhibited a significant reduction in serum glucose, total cholesterol, triglycerides, LDLcholesterol and increase in HDL-cholesterol, Plasma insulin in alloxan induced diabetic rats. The antidiabetic effect of AEMC was similar to Glibenclamide. Hence our study reveals the antidiabetic and Hypolipidemic potential of Mimosa catechu bark and the study could be helpful to develop medicinal preparations for diabetes and related symptoms.

Keywords

Mimosa catechu, Hypoglycemic Activity, Hypolipidemic Activity, Alloxan, Glibenclamide.

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B. Thangabalan ¹, Ch. Sandhya ¹, N. Sunitha ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A simple, fast and precise stability indicating RP - HPLC method was developed for the quantification of Memantine HCl in pure and pharmaceutical dosage form. The quantification was carried out using Intersil ODS C18 4.6 X 150mm, 5μm enhanced polar selectivity column and mobile phase comprised of 0.2M sodium dihydrogen phosphate&0.1M disodium hydrogen phosphate buffer of pH adjusted to 3 with orthophosphoric acid and Acetonitrile in proportion of ratio 50:50 and degassed under ultrasonication. The flow rate was 1ml/min and the effluent was monitored at 272nm. The retention time of Memantine HCl was found to be 3.337 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Memantine was in the range of 20 - 120 μg/mL. The percentage recoveries of Memantine were 99.66% to 102.5% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Memantine HCl in pharmaceutical dosage form.

Keywords

Chromatography, Memantine HCI, Method Validation, Forced Degradation Study.

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